Welcome to your MLT- Professional Name: Email: Phone Number: [Cold enrichment of feces (incubation at 4°C) in phosphate-buffered saline prior to subculture onto enteric media enhances the recovery of:] Enterotoxigenic E. coli Salmonella paratyphi Hafnia alvei Y. enterocolitica None . [Cetrimide agar is used as a selective isolation agar for which organism?] Acinetobacter spp. Pseudomonas aeruginosa Moraxella spp. Stenotrophomonas maltophilia None . [Microscopic examination of a fungus cultured from a patient with athlete’s foot showed large, smooth-walled, club-shaped macroconidia appearing singly or in clusters of two to three from the tips of short conidiophores. The colonies did not produce microconidia. What is the most likely identification?] Trichophyton spp. Alternaria spp. Epidermophyton spp. Microsporum spp. None . [A CSF sample obtained from a 2-week old infant with suspected bacterial meningitis grew gram-negative rods on blood and chocolate agars. The following results were noted: MacConkey agar = No growth ONPG = + Glucose (open) OF = + Urease = Neg Glucose (closed) OF = Neg Catalase = + Indole = + Oxidase = + Motility = Neg Pigment = Yellow 42° C growth = Neg What is the correct identification?] Pseudomonas aeruginosa Chryseobacterium meningosepticum Acinetobacter baumannii E. coli None . [What is the clinical significance of K-ras testing?] K-Ras mutations make tumor cells more susceptible to chemotherapy K-Ras is a tumor suppressor gene and mutations are associated with increased lifetime risk of malignancy K-Ras mutations result in treatment resistance to growth factor receptor inhibitors K-Ras is used to identify the tissue of origin None . [Why is a chemiluminescent immunoassay (CIA) or enzyme immunoassay (EIA) the method of choice for detection of certain analytes, such as hormones, normally found in low concentrations?] Because of low cross reactivity Because of high specificity Because of high sensitivity Because test systems may be designed as both competitive and noncompetitive assays None . [An inpatient is eating breakfast when you arrive to collect a fasting glucose. What is the best thing to do?] Consult with the patient’s nurse to see if the specimen should be collected. Draw the specimen quickly before the patient finishes eating. Draw the specimen and write “non fasting” on the requisition. Fill out a form stating the patient had eaten so the specimen was not drawn. None . [Which crystal appears in urine as a long, thin hexagonal plate, and is linked to ingestion of large amounts of benzoic acid?] Cystine Hippuric acid Oxalic acid Uric acid None . [β-Hemolytic streptococci, more than 50,000 col/mL, were isolated from a urinary tract catheter urine specimen. Given the following reactions, what is the most likely identification? CAMP test = Neg Hippurate hydrolysis = +-} Bile solubility = Neg 6.5% salt = + PYR = + Bile esculin = + SXT = Resistant Bacitracin = Resistant Optochin = Resistant] Group A streptococci Group B streptococci Enterococcus faecalis Nongroup A, nongroup B, nongroup D streptococci None . [An Entamoeba histolytica trophozoite has the following characteristics:] Central karyosome in the nucleus, ingested RBCs, and clear pseudopodia Ingested RBCs, clear pseudopodia, and uneven chromatin on the nuclear membrane Ingested RBCs, clear pseudopodia, and large glycogen vacuoles in cytoplasm Large, blotlike karyosome, ingested white blood cells (WBCs), and granular pseudopods None . [The reference method for serum lipase is based upon:] Assay of triglycerides following incubation of serum with olive oil Rate turbidimetry Titration of fatty acids with dilute NaOH following controlled incubation of serum with olive oil Immunochemical assay None . [Calculate the mean cell hemoglobin concentration (MCHC) using the following values:Hgb: 15 g/dL (150 g/L) Hct: 47 mL/dL (0.47)RBC: 4.50 × 10ˆ6/μL (4.50 × 10¹²/L)] 9.5% (.095) 10.4% (.104) 31.9% (.319) 33.3% (.333) None Comment . [Given the following real-time PCR amplification curve, what is the threshold cycle?(fig)] 15 20 25 30 None Comment . [Which test typically requires a refrigerated stool specimen?] Fecal fat Guaiac Occult blood Ova and parasites None . [What is an advantage of performing a prostatespecific antigen (PSA) test for prostate cancer?] PSA is stable in serum and not affected by a digital-rectal examination PSA is increased only in prostatic malignancy A normal serum level rules out malignant prostatic disease The percentage of free PSA is elevated in persons with malignant disease None . [Which of the following statements regarding the Philadelphia chromosome is true?] It is seen exclusively in chronic myelogenous leukemia It results from a translocation It appears as a short-arm deletion of chromosome 21 It is associated with a poor prognosis None . [Plate 2 shows the electrophoresis of serum proteins on a high-resolution agarose gel atpH 8.6. Sample 1 (in lane 1) is a normal serum control. Which sample can be presumptively classified as a monoclonal gammopathy?] Sample 2 Sample 4 Sample 6 Sample 8 None Comment . [An autologous unit of whole blood was collected on a 33-year-old woman in preparation for a knee replacement procedure in 3 weeks. The whole blood unit had her hyphenated last name, first name, and last four digits of her social security number for identification. The lab computer system, however, only had her married name and first name, medical record number, and social security number. What should be done with this blood product?] Discard the unit Make the unit available for transfusion Confirm the name with donor and have admissions make the correction in the computer system, then make the unit available for transfusion Ensure that social security numbers match, confirm the name with donor and have admissions make the correction in the computer system with the medical director’s approval, then make the unit available for transfusion None . [Plate 37 is a Wright’s-stained peripheral blood film, 1,000×. The cells seen in this plate are associated with:] Lead poisoning Aplastic anemia Iron deficiency anemia Intravascular hemolysis None Comment . [Bacitracin A disks (0.04 unit) are used for the presumptive identification of which group of β-hemolytic streptococci?] Group A Group B Group C Group F None . [Informed consent means that a:] nurse has the right to perform a procedure on a patient even if the patient refuses. patient agrees to a procedure after being told of the consequences associated with it. patient has the right to look at all his or her medical records and test results. phlebotomist tells the patient what is ordered and the implications of the test results. None . [Which media should be used to recover Bordetella pertussis from a nasopharyngeal specimen?] Chocolate agar Blood agar MacConkey agar Bordet–Gengou agar None . [What is the recommended troponin T and I cutoff (upper limit of normal) for detecting myocardial infarction?] The cutoff varies with the method of assay but should be no lower than 0.2 ng/mL The upper 99th percentile or lowest level that can be measured with 10% CV The concentration corresponding to the lowest level of calibrator used The highest value fitting under the area of the curve for the 95% confidence interval None . [Which of the following most affects the oxidation–reduction potential (Eh or redox potential) of media for anaerobic bacteria?] O2 Nitrogen pH Glucose None . [The familial condition of Pelger–Huet anomaly is important to recognize because this disorder must be differentiated from:] Infectious mononucleosis May–Hegglin anomaly A shift-to-the-left increase in immature granulocytes G6PD deficiency None . [Which of the following conditions characterizes chronic glomerulonephritis and helps to differentiate it from acute glomerulonephritis?] Hematuria Polyuria Hypertension Azotemia None . [Patient HistoryA 3-year-old male was admitted to a hospital with scattered petechiae and epistaxis. The patient had normal growth and had no other medical problems except for chickenpox 3 weeks earlier. His family history was unremarkable.Laboratory Results Patient Reference RangePT: 11 sec 10–13 secAPTT: 32 sec 28–37 secPlatelet count: 18 × 10^3/μL 150–450 × 10^3/μLThese clinical manifestations and laboratory results are consistent with which condition?] TTP DIC ITP HUS None Comment . [Which of the following reagents is added to detect the production of indole?] p-Dimethylaminobenzaldehyde Bromcresol purple Methyl red Cytochrome oxidase None . [Which region of the immunoglobulin molecule can bind antigen?] Fab Fc CL CH None . [A delta check refers to:] checking the wristband with the requisition. comparing current test results with previous one. documenting all of the results of the QC checks. reporting new infection control precautions. None . Time's up
